文章基本信息

标题：Functional physiological phenotyping with functional mapping: A general framework to bridge the phenotype-genotype gap in plant physiology
本地全文：下载
作者：Arun K. Pandey ; Libo Jiang ; Menachem Moshelion 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：8
页码：1-14
DOI：10.1016/j.isci.2021.102846
语种：English
出版社：Elsevier
摘要：SummaryThe recent years have witnessed the emergence of high-throughput phenotyping techniques. In particular, these techniques can characterize a comprehensive landscape of physiological traits of plants responding to dynamic changes in the environment. These innovations, along with the next-generation genomic technologies, have brought plant science into the big-data era. However, a general framework that links multifaceted physiological traits to DNA variants is still lacking. Here, we developed a general framework that integrates functional physiological phenotyping (FPP) with functional mapping (FM). This integration, implemented with high-dimensional statistical reasoning, can aid in our understanding of how genotype is translated toward phenotype. As a demonstration of method, we implemented the transpiration and soil-plant-atmosphere measurements of a tomato introgression line population into the FPP-FM framework, facilitating the identification of quantitative trait loci (QTLs) that mediate the spatiotemporal change of transpiration rate and the test of how these QTLs control, through their interaction networks, phenotypic plasticity under drought stress.Graphical abstractDisplay OmittedHighlights•FPP-FM allows real-time depicting of genetic interactions in response to environment•It estimates genetic effects from physiological and developmental principles of growth•FPP-FM serves the translation of physiological data into genetic gainPlant genetics; Plant biology; Omics