文章基本信息

标题：NANOS2 suppresses the cell cycle by repressing mTORC1 activators in embryonic male germ cells
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作者：Ryuki Shimada ; Hiroko Koike ; Takamasa Hirano 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：8
页码：1-25
DOI：10.1016/j.isci.2021.102890
语种：English
出版社：Elsevier
摘要：SummaryDuring murine germ cell development, male germ cells enter the mitotically arrested G0 stage, which is an initial step of sexually dimorphic differentiation. The male-specific RNA-binding protein NANOS2 has a key role in suppressing the cell cycle in germ cells. However, the detailed mechanism of how NANOS2 regulates the cell cycle remains unclear. Using single-cell RNA sequencing (scRNA-seq), we extracted the cell cycle state of each germ cell in wild-type andNanos2-KO testes and revealed thatNanos2expression starts in mitotic cells and induces mitotic arrest. We identifiedRheb, a regulator of mTORC1, andPtmaas possible targets of NANOS2. We propose that repression of the cell cycle is a primary function of NANOS2 and that it is mediated via the suppression of mTORC1 activity through the repression ofRhebin a post-transcriptional manner.Graphical abstractDisplay OmittedHighlights•Single-cell RNA-seq data recapitulated sexual differentiation processes•Cell cycle state of developing germ cells was revealed by single-cell RNA-seq data•NANOS2 suppresses mTORC1 activators to induce cell cycle arrest of germ cellsBiological sciences; Molecular biology; Developmental biology