文章基本信息

标题：The Effects of Resveratrol Treatment on Bcl-2 and Bax Gene Expression in Endometriotic Compared with Non-Endometriotic Stromal Cells
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作者：Roya KOLAHDOUZ-MOHAMMADI ; Ali-Akbar DELBANDI ; Sepideh KHODAVERDI 等
期刊名称：Iranian Journal of Public Health
印刷版ISSN：2251-6085
电子版ISSN：2251-6093
出版年度：2020
卷号：49
期号：8
DOI：10.18502/ijph.v49i8.3900
语种：English
出版社：Tehran University of Medical Sciences
摘要：Background: We aimed to examine resveratrol effects on gene expression of Bcl-2, Bax and Bcl-2ax ratio in endometrial stromal cells derived from women with and without endometriosis. Methods: Endometrial tissues were obtained from 40 endometriotic patients and 15 non-endometriotic controls undergoing laparoscopic surgery or hysterectomy in the gynecology ward of Rassoul Akram Hospital, Tehran, Iran from 2015 to 2017. After the enzymatic digestion, eutopic (EuESCs) and ectopic (EESCs) endometrial stromal cells from patients with endometriosis as well as endometrial stromal cells from non-endometriotic controls (CESCs) were treated with or without resveratrol (100 µM) and the levels of Bcl-2, Bax and Bcl-2ax gene expression ratio in the cells from all origins were examined at 6, 24 and 48 h post-treatment by real-time PCR. Results: Resveratrol treatment increased Bcl-2 expression in CESCs at 24 and 48 h and in EuESCs at 48 h (P0.05), but had no significant effects on the expression of this gene in EESCs. On the other hand, resveratrol treatment increased Bax expression in EuESCs at 6 h and decreased its expression in EESCs at 48 h (P0.05). Regarding the Bcl-2ax gene expression ratio, resveratrol treatment increased Bcl-2ax gene expression ratio in CESCs and EuESCs at 48 h (P0.01). However, this treatment had no significant differential effect on Bcl-2 and Bcl-2ax gene expression ratio between CESCs and EuESCs at 48 h. Conclusion: Resveratrol treatment significantly increased Bcl-2ax gene expression ratio in EuESCs and CESCs but had no significant effect in EESCs.