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标题：Characterization and visualization of murine coagulation factor VIII-producing cells in vivo
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作者：Morisada Hayakawa ; Asuka Sakata ; Hiroko Hayakawa 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2021
卷号：11
DOI：10.1038/s41598-021-94307-0
语种：English
出版社：Springer Nature
摘要：Coagulation factors are produced from hepatocytes, whereas production of coagulation factor VIII (FVIII) from primary tissues and cell species is still controversial. Here, we tried to characterize primary FVIII-producing organ and cell species using genetically engineered mice, in which enhanced green fluorescent protein (EGFP) was expressed instead of the F8 gene. EGFP-positive FVIII-producing cells existed only in thin sinusoidal layer of the liver and characterized as CD31 high, CD146 high, and lymphatic vascular endothelial hyaluronan receptor 1 (Lyve1) +. EGFP-positive cells can be clearly distinguished from lymphatic endothelial cells in the expression profile of the podoplanin − and C-type lectin-like receptor-2 (CLEC-2) +. In embryogenesis, EGFP-positive cells began to emerge at E14.5 and subsequently increased according to liver maturation. Furthermore, plasma FVIII could be abolished by crossing F8 conditional deficient mice with Lyve1-Cre mice. In conclusion, in mice, FVIII is only produced from endothelial cells exhibiting CD31 high, CD146 high, Lyve1 +, CLEC-2 +, and podoplanin − in liver sinusoidal endothelial cells.