摘要:The timing and motivation of prehistoric human expansion into the hinterland of the Tibetan Plateau (TP) is a widely debated scientific issue. Recent archaeological studies have brought forward predictions of the earliest human occupation of the TP to the late–Middle Pleistocene. However, massive human occupation of the TP did not appear until the termination of the Last Glacial Maximum (LGM). The spatio-temporal distribution of prehistoric hunter-gatherers on the TP varies significantly before the permanent occupation after 3600 BP (before present). Here, we report on environmental-archaeological evidence from the Canxionggashuo (CXGS) site in Yushu Prefecture, which provides information that is key to understanding the dynamics of post-LGM human occupation on the TP. Radiocarbon dating has revealed two occupation periods of the CXGS site at 8600–7100 cal (calibrated years) BP and 2400–2100 cal BP. The charcoal concentration in cultural layers correlates well with paleo–human activities. Hunter-gatherers expanded westwards from the northeastern margin of the TP to the hinterland of the TP during the warming period of the early–middle Holocene (~11,500–6000 BP). However, these groups retreated during the middle–late Holocene (~6000–3600 BP) under a cooling-drying climate. Prehistoric humans finally occupied the hinterland of the TP permanently after 3600 BP, with an enhanced cold-adaptive lifestyle, although the climate was still deteriorating.