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标题：A photoswitchable helical peptide with light-controllable interface/transmembrane topology in lipidic membranes
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作者：Mónica Gutiérrez-Salazar ; Eduardo Santamaría-Aranda ; Louise Schaar 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：7
页码：1-23
DOI：10.1016/j.isci.2021.102771
语种：English
出版社：Elsevier
摘要：SummaryThe spontaneous insertion of helical transmembrane (TM) polypeptides into lipid bilayers is driven by three sequential equilibria: solution-to-membrane interface (MI) partition, unstructured-to-helical folding, and MI-to-TM helix insertion. A bottleneck for understanding these three steps is the lack of experimental approaches to perturb membrane-bound hydrophobic polypeptides out of equilibrium rapidly and reversibly. Here, we report on a 24-residues-long hydrophobic α-helical polypeptide, covalently coupled to an azobenzene photoswitch (KCALP-azo), which displays a light-controllable TM/MI equilibrium in hydrated lipid bilayers. FTIR spectroscopy reveals thattransKCALP-azo folds as a TM α-helix (TM topology). Aftertrans-to-cisphotoisomerization of the azobenzene moiety with UV light (reversed with blue light), the helical structure of KCALP-azo is maintained, but its helix tilt increased from 32 ± 5° to 79 ± 8°, indication of a reversible TM-to-MI transition. Further analysis indicates that this transition is incomplete, withcisKCALP-azo existing in a ∼90% TM and ∼10% MI mixture.Graphical abstractDisplay OmittedHighlights•We present an α-helical transmembrane peptide modified with a molecular photoswitch•The peptide exhibits reversible photocontrol of its membrane topology•A fraction moves to the membrane interface with UV and inserts back with blue light•This system will be useful to address the molecular mechanism for membrane insertionPhotoabsorption; Biomolecules; Membranes