文章基本信息

标题：Gut probiotic bacteria of Barbonymus gonionotus improve growth, hematological parameters and reproductive performances of the host
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作者：Mohammad Abdus Salam ; Md. Ariful Islam ; Sulav Indra Paul 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2021
卷号：11
DOI：10.1038/s41598-021-90158-x
语种：English
出版社：Springer Nature
摘要：This study aimed to isolate and identify probiotic bacteria from the gut of Barbonymus gonionotus and evaluate their effects on growth, hematological parameters, and breeding performances of the host. Five probiotic bacteria viz . Enterococcus xiangfangensis (GFB-1), Pseudomonas stutzeri (GFB-2), Bacillus subtilis (GFB-3), Citrobacter freundii (GFB-4), and P. aeruginosa (GFB-5) were isolated and identified using 16S rRNA gene sequencing. Application of a consortium of probiotic strains (1–3 × 1.35 × 10 9 CFU kg −1) or individual strain such as GFB-1 (1.62 × 10 9 CFU kg −1), GFB-2 (1.43 × 10 9 CFU kg −1), GFB-3 (1.06 × 10 9 CFU kg −1), GFB-4 (1.5 × 10 9 CFU kg −1) or GFB-5 (1.43 × 10 9 CFU kg −1feed) through feed significantly improved growth, histological and hematological parameters and reproductive performances of B. gonionotus compared to untreated control. Moreover, the application of these probiotics significantly increased gut lactic acid bacteria and activities of digestive enzymes but did not show any antibiotic resistance nor any cytotoxicity in vitro. The highest beneficial effects on treated fishes were recorded by the application of GFB-1, GFB-2, GFB-3, and a consortium of these bacteria (T2). This is the first report of the improvement of growth and health of B. gonionotus fishes by its gut bacteria.