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标题：MOLECULAR IDENTIFICATION OF PHYTOPLASMAGROUPS IN ALWIJAM-AFFECTED DATE PALMS IN SAUDI ARABIA AND JORDAN
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作者：Khalid A Alhudaib ; Adel A Rezk ; Abeer Abushribi 等
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2021
卷号：30
期号：2
页码：1335-1343
语种：English
出版社：PSP Publishing
摘要：Date palm,(Phoenix dactylifera L.) is a valuable fruit crop in the Middle East including Saudi Arabia and Jordan.The phytoplasma (Alwijam) disease,caused by Candidtus phytoplasma,is considered one of the most important diseases that affect the sustainability of date palm.One thousand and fifty seven date palm samples were collected from different locations in Saudi Arabia and Jordan and tested for Alwijam using nested polymerase chain reaction (nPCR) with sets of primers P1/P7 and R16F2n/R16R2.The percentage of occurrence of phytoplasma in the collected samples were 11.3% in Saudi Arabia and 8.4% in Jordan.Nucleotide sequences for 16S rRNA gene of obtained isolates were carried out and virtual Restriction Fragment Length Polymorphism RFLP patterns analysis were used to study the diversity of the pathogen that causing Alwijam disease in both countries.Phytoplasma groups 16SrI,16SrII and 16SrV reported to be associated with Alwijam disease on date palm in both countries.This study has demonstrated that Alwijam disease identified for first time in Jordan and the group 16SrV reported for first time associated with Alwijam disease.
关键词：Alwijam;Date palm;Candidtus phytoplasma;nPCR;RFLP