文章基本信息

标题：Macrophage recruitment in immune-privileged lens during capsule repair, necrotic fiber removal, and fibrosis
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作者：Yuting Li ; Zhen Li ; Yumeng Quan 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：6
页码：1-21
DOI：10.1016/j.isci.2021.102533
语种：English
出版社：Elsevier
摘要：SummaryEmerging evidence challenges the lens as an immune-privileged organ. Here, we provide a direct mechanism supporting a role of macrophages in lens capsule rupture repair. Posterior lens capsule rupture in a connexin 50 and aquaporin 0 double-knockout mouse model resulted in lens tissue extrusion into the vitreous cavity with formation of a “tail-like” tissue containing delayed regressed hyaloid vessels, fibrotic tissue and macrophages at postnatal (P) 15 days. The macrophages declined after P 30 days with M2 macrophages detected inside the lens. By P 90 days, the “tail-like” tissue completely disappeared and the posterior capsule rupture was sealed with thick fibrotic tissue. Colony-stimulating factor 1 (CSF-1) accelerated capsule repair, whereas inhibition of the CSF-1 receptor delayed the repair. Together, these results suggest that lens posterior rupture leads to the recruitment of macrophages delivered by the regression delayed hyaloid vessels. CSF-1-activated M2 macrophages mediate capsule rupture repair and development of fibrosis.Graphical abstractDisplay OmittedHighlights•Lens posterior rupture delays regression of the hyaloid vessels.•Lens posterior rupture recruits macrophages delivered by the hyaloid vessels.•Macrophages mediate necrotic fiber cell removal and capsule rupture sealing.•CSF-1 activated M2 macrophages facilitate capsular rupture sealing by fibrosis.Immunology; Ophthalmology