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标题：Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish
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作者：Pedro Perdiguero ; Esther Morel ; Patricia Díaz-Rosales 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：6
页码：1-19
DOI：10.1016/j.isci.2021.102615
语种：English
出版社：Elsevier
摘要：SummaryB cells express a unique antibody protein which comprises two pairs of immunoglobulin (Ig) heavy (H) and light (L) chains. In addition to an invariable constant (C) region, IgH and IgL chains encompass a variable (V) region mediating antigen binding. This unique region stems from Ig V(D)J gene recombination, which generates diversity by assembling these gene segments into VHDJHand VLJLgenes. To ensure that one B cell only expresses one antibody, VHDJHrearrangement occurs only in one IgH locus (allelic exclusion), whereas VLJLrearrangement only in either the κ or λ locus (isotype exclusion). However, teleosts express multiple IgLs encoded by distinct CLgenes. Using single-cell transcriptomics, we have demonstrated the transcription of distinct rearranged VLJLCLgenes in single rainbow trout B cells. Our results highlight the laxity of isotype exclusion in teleosts and strongly suggest that fish B cells can produce antibodies of different specificities.Graphical abstractDisplay OmittedHighlights•Single teleost B cells transcribe different IgL chain sub-isotypes•In many cells, more than one transcript is productively rearranged as VLJLCL•These differently rearranged VLJLCLgenes render IgL chains with variable CDR3•This suggests that single fish B cells can produce Igs of different specificitiesImmunology; Cell biology; Transcriptomics