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标题：Deep hypometabolic state mitigates radiation-induced lethality in zebrafish
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作者：Subhajit Ghosh ; Namita Indracanti ; Jharna Ray 等
期刊名称：Biomedical Research and Clinical Practice
电子版ISSN：2397-9631
出版年度：2017
卷号：2
期号：3
页码：1-9
DOI：10.15761-BRCP.1000139
语种：English
出版社：Open Access Text
摘要：The increased risks of ionizing radiation exposure both due to accidental and malevolent terrorist acts demand efficient prophylactics and mitigators. As of now there are no clinically safe and effective radiomitigators. Deep hypometabolic (DH) or suspended animation (SA)-like state render organism’s resistant to a variety of stresses. The present study aims at investigating the radiomitigative effect of anoxia-mediated DH state using developing zebrafish as a vertebrate model. Anoxia efficiently induced a DH state (heartbeat, movement and developmental arrest) in developing zebrafish and effects reversed when the embryos brought back to a normoxic environment. Embryos of 4 hpf (hours post-fertilization) in the hypometabolic state effectively tolerated the effects of supra-lethal doses (20 Gy; LD80/6) gamma radiation and rescued from radiation-induced pericardial edema, microopthlamia, microcephaly, skin roughness, bradycardia and lethality (80% survival advantage after 6 days, over zero survival in radiation treatment alone). The DH-like state induced immediately after exposure to 20 Gy gamma radiation delayed as well as minimized the damage manifestations, and the extent of the delay was found to be equal to the duration for which the embryos were in DH state. The present study demonstrates that the DH state protects zebrafish from radiation-induced lethality and also mitigates radiation-induced damaging effects when induced prior to or after lethal radiation exposures, respectively.
关键词：radiation; hypometabolic state; radiomitigation; zebrafish; suspended animation