摘要:Tea tree oil (TTO) from the genus <i>Melaleuca</i> L. has antimicrobial, antibacterial, antifungal, and antioxidant properties and is used by the cosmetic, pharmaceutical, and horticultural industries. In Pakistan, <i>Melaleuca bracteata</i> can be exploited for essential oil purposes, as this species is well adapted to Pakistan’s agroclimatic conditions. The objective of the present study was to evaluate the yield of <i>M. bracteata</i> essential oil together with its antioxidant and antimicrobial properties under local prevailing conditions of the subtropics. Essential oil was extracted through the hydrodistillation method. Using this method, six batches of 8 kg samples (fresh leaves and branches) underwent a distillation process for 4–5 h. The average yield obtained was about 0.2%. The GCMS was used to identify the components of extracted essential oil. Eugenol methyl ether is the major component in extracted essential oil, i.e., 96% of the total. A high content of flavonoids and phenolics and a Fe-reducing power ability of <i>M. bracteata</i> were observed. The oil was also found effective against <i>B. subtilis</i>, <i>B. cereus</i>, White rot, and <i>A. flavus</i>. Hence, it is concluded that there is a possibility to use TTO for its biocidal properties, and it must also be inspected and then commercialized in Pakistan by the agriculture and cosmetic industries.