文章基本信息

标题：FoldAffinity: binding affinities from nDSF experiments
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作者：Stephan Niebling ; Osvaldo Burastero ; Jérôme Bürgi 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2021
卷号：11
DOI：10.1038/s41598-021-88985-z
语种：English
出版社：Springer Nature
摘要：Differential scanning fluorimetry (DSF) using the inherent fluorescence of proteins (nDSF) is a popular technique to evaluate thermal protein stability in different conditions (e.g. buffer, pH). In many cases, ligand binding increases thermal stability of a protein and often this can be detected as a clear shift in nDSF experiments. Here, we evaluate binding affinity quantification based on thermal shifts. We present four protein systems with different binding affinity ligands, ranging from nM to high μM. Our study suggests that binding affinities determined by isothermal analysis are in better agreement with those from established biophysical techniques (ITC and MST) compared to apparent K d s obtained from melting temperatures. In addition, we describe a method to optionally fit the heat capacity change upon unfolding ( \documentclass[12pt