文章基本信息

标题：Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata
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作者：S S Jayaraj ; R Thiagarajan ; M Arumugam 等
期刊名称：Invertebrate Survival Journal
电子版ISSN：1824-307X
出版年度：2010
卷号：7
期号：1
页码：79-88
语种：English
出版社：Universita degli Studi di Modena e Reggio Emilia
摘要：A naturally occurring hemagglutinin (HA), with activity against bacteria and yeast cells weredetected in the serum of Scylla serrata using mammalian erythrocytes (RBC), various bacteria and yeast as indicator cells. The serum gave highest HA titer with rabbit RBC, tripsinized yeast and Vibrio fluvialis. An analysis of the physico-chemical properties of the HA showed it to be specifically dependent on the presence of Ca2+ for its activity, stable between pH 7 to 9 and showed thermal stability between 10 to 30 °C. Further studies demonstrated that the HA is precipitable by ammonium sulphate and TCA. HA- inhibition assays performed with carbohydrates revealed that the serum HA was specific for non-reducing terminal glucose with 1-2 glucosidic linkages. Thus this agglutinin appears to be unique among all the known crustacean agglutinins.
关键词：hemagglutinin;bacterial agglutinin;yeast agglutinin;serum;mud crab;Scylla serrata