文章基本信息

标题：Effects of fluoride on primary cultured haemocytes from the marine gastropod Haliotis tuberculata
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作者：R Ladhar-Chaabouni ; T Houel ; J M Lebel 等
期刊名称：Invertebrate Survival Journal
电子版ISSN：1824-307X
出版年度：2019
卷号：16
期号：1
页码：1-7
DOI：10.25431/1824-307X/isj.v0i0.1-7
语种：English
出版社：Universita degli Studi di Modena e Reggio Emilia
摘要：As a consequence of human’s activities, fluoride concentration in many aquatic ecosystems is significantly increasing. Nevertheless, little is known about fluoride toxicity to aquatic life. In this study the effect of exposure to different concentrations of sodium fluoride (2, 10, 50, 250 and 1,250 μg mL−1) during 24 h on primary cultured haemocytes of the gastropod Haliotis tuberculata was realized. Results indicate no significant effect of NaF on cell viability, Lysosomal membrane stability, phagocytosis and ROS production at concentrations of 2, 10, 50 and 250 μg mL−1. Nevertheless, lysosomal membrane alterations, a decrease of phagocytosis and morphological changes of H. tuberculata haemocytes were observed at concentration of 1,250 μg mL−1 NaF suggesting a potential impact of NaF at high concentration in the environment.
关键词：Fluoride;Haemocytes;Haliotis tuberculata;Immune parameters;In vitro;Primary culture