文章基本信息

标题：Identification of cAMP-dependent phosphorylated proteins involved in the formation of environment-resistant resting cysts by the terrestrial ciliate Colpoda cucullus
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作者：Y Sogame ; K Kojima ; T Takeshita 等
期刊名称：Invertebrate Survival Journal
电子版ISSN：1824-307X
出版年度：2014
卷号：11
期号：1
页码：213-218
语种：English
出版社：Universita degli Studi di Modena e Reggio Emilia
摘要：In the terrestrial ciliate Colpoda cucullus, an elevation of the intracellular cAMP concentration was reported to be involved in environment-resistant resting cyst formation. In the present study, cAMP-dependently phosphorylated proteins of encystment-induced C. cucullus were isolated with Phos-tag agarose phosphate-affinity beads and subsequent SDS-PAGE. In a liquidchromatography/tandem mass spectrometry analysis of these phosphoproteins, 27-, 37- and 43-kDa proteins (p27, p37 and p43) were identified as Rieske iron-sulfur protein, histone H4 (hyperacetylated form), and actin, respectively.
关键词：environment-resistant cyst;Colpoda;encystment;protein phosphorylation;cAMP