文章基本信息

标题：Volume-constrained microcontainers enable myoepithelial functional differentiation in highly parallel mammary organoid culture
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作者：Michael E. Todhunter ; Masaru Miyano ; Divya S. Moolamalla 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：4
页码：1-30
DOI：10.1016/j.isci.2021.102253
语种：English
出版社：Elsevier
摘要：SummaryA long-standing constraint on organoid culture is the need to add exogenous substances to provide hydrogel matrix, which limits the study of fully human or fully native organoids. This paper introduces an approach to culture reconstituted mammary organoids without the impediment of exogenous matrix. We enclose organoids in nanoliter-scale, topologically enclosed, fluid compartments surrounded by agar. Organoids cultured in these “microcontainers” appear to secrete enough extracellular matrix to yield a self-sufficient microenvironment without exogenous supplements. In microcontainers, mammary organoids exhibit contractility and a high-level, physiological, myoepithelial (MEP) behavior that has not been previously reported in reconstituted organoids. The presence of contractility suggests that microcontainers elicit MEP functional differentiation, an important milestone. Microcontainers yield thousands of substantially identical and individually trackable organoids within a single culture vessel, enabling longitudinal studies and statistically powerful experiments, such as the evaluation of small effect sizes. Microcontainers open new doors for researchers who rely on organoid models.Graphical abstractDisplay OmittedHighlights•Microcontainers are volume-constrained microwells with hydrogel lids•Microcontainers enable statistically robust experimental design with organoids•Organoids produce their own extracellular matrix within microcontainers•Myoepithelial cells in mammary organoids achieve fully functional differentiationCell Biology; Stem Cells Research; Bioengineering; Tissue Engineering