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标题：Tannin acyl-hydrolase production by Bacillus subtilis KMS2-2: Purification, characterization, and cytotoxicity studies
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作者：Rasiravathanahalli Kaveriyappan Govindarajan ; Krishnamurthy Mathivanan ; Chartchai Khanongnuch 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2021
卷号：33
期号：3
页码：1-9
DOI：10.1016/j.jksus.2021.101359
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractObjectiveBacterial mediated tannin acyl-hydrolase production was investigatedBacillus subtilisKMS2-2 in this study. Tannin acyl-hydrolase used as catalysts in the production of glucose and gallic acid. Also, it has potential application in beverage and food processing.MethodsThis bacterial TAH enzyme was purified by asingle methods approach consisting of size exclusion chromatography (sephadex G-100) and characterization of purified enzyme using different methods followed.ResultThe molecular mass of purified TAH was determined as (~43 kDa) on 12% (PAGE) and it was confirmed by (MALDI-TOF/MS). Characterization of purified tannin acyl-hydrolase by (HPLC) confirmed that gallic acid was formed as a by-product during hydrolysis of tannic acid as a substrate and (FT-IR) spectroscopy showed the functional groups such as O–H, C-O and C–C. The purified tannin acyl-hydrolase retained the enzyme activity upto 80% under the conditions at 50 °C and pH 6.0 after 60 min incubation. The TAH comprises a typical secondary structure at pH 6.0 and contains α-(16.8%), β-(45.1%), and Turns (38.4%). Also, thermodynamics parameters and different additives like inhibitors, chelators, and metal ions were studied on the tannin acyl hydrolase. Further, the purified TAH enzyme has no cytotoxicity on the vero cell line as well as rat model study.ConclusionFrom this experiment, theB. subtilisKMS2-2 would provide a possible resource for more efficient tannin-acyl hydrolase production and can be used for various industrial purposes, in particular food, feed, pharmaceutical industry.
关键词：Bacillus subtilis;Bacterial Tannin acyl-hydrolase (TAH);Sephadex G-100;SDS-PAGE;In vivo;model