文章基本信息

标题：Optimizing bacterial resource allocation: metabolite production in continuous bioreactors ⁎
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作者：Agustín Gabriel Yabo ; Jean-Luc Gouzé
期刊名称：IFAC PapersOnLine
印刷版ISSN：2405-8963
出版年度：2020
卷号：53
期号：2
页码：16753-16758
DOI：10.1016/j.ifacol.2020.12.1146
语种：English
出版社：Elsevier
摘要：AbstractWe show preliminary results addressing the problem of resource allocation in bacteria in the continuous bioreactor framework. We propose a coarse-grained self-replicator dynamical model that accounts for the microbial population growth inside a continuous bioreactor, and we study its asymptotic behavior. This is done through a dynamical systems analysis approach, in order to provide conditions for the persistence of the bacterial population. We then study the two most relevant cases of steady-state production in this scheme: 1) biomass production, classical in high-tech industrial processes as well as in research environments; and 2) metabolite production through the introduction of a heterologous metabolic pathway. Both problems are explored in terms of the internal allocation control—which can be externally disrupted—and the constant volumetric flow of the bioreactor; and analyzed through a numerical approach. The resulting two-dimensional optimization problem is defined in terms of Michaelis-Menten kinetics using the parameter values of previous works, and taking into account the constraints for the existence of the equilibrium of interest.
关键词：Keywordsdynamicscontrolmodelingidentificationindustrial biotechnology