摘要:(1) Aims: This study was aimed to investigate the effects of organic and inorganic fertilizer application on the soil nutrients and microbiota in tea garden soil. (2) Method: Illumina Hiseq sequencing technique was conducted to analyze the microbial diversity and density in different fertilizer-applied tea garden soil. (3) Results: The results showed that <i>Acidobacteria</i>, <i>Proteobacteria</i> and <i>Actinobacteria</i> were the predominant bacterial species observed in the tea garden soil. Besides, the relative abundance of <i>Basidiomycota</i>, <i>Ascomycota</i> and <i>Zygomycota</i> fungal species were higher in the tea garden soil. Correlation analysis revealed that <i>Acidibacter</i> and <i>Acidothermus</i> were significantly correlated with chemical properties (such as total organic carbon (TOC), total phosphorus (TP) and available phosphorus (AP) contents) of the tea garden soil. Furthermore, all these microbes were abundant in medium rapeseed cake (MRSC) + green manure (GM) treated tea garden soil. (4) Conclusion: Based on the obtained results, we conclude that the application of MRSC + GM could be a preferred fertilizer to increase the soil nutrients (TOC, TP and AP content) and microbial population in the tea garden soil.