文章基本信息

标题：Single-cell RNA-seq analysis reveals compartment-specific heterogeneity and plasticity of microglia
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作者：Junying Zheng ; Wenjuan Ru ; Jay R. Adolacion 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：3
页码：1-25
DOI：10.1016/j.isci.2021.102186
语种：English
出版社：Elsevier
摘要：SummaryMicroglia are ubiquitous central nervous system (CNS)-resident macrophages that maintain homeostasis of neural tissues and protect them from pathogen attacks. Yet, their differentiation in different compartments remains elusive. We performed single-cell RNA-seq to compare microglial subtypes in the cortex and the spinal cord. A multi-way comparative analysis was carried out on samples from C57/BL and HIV gp120 transgenic mice at two, four, and eight months of age. The results revealed overlapping but distinct microglial populations in the cortex and the spinal cord. The differential heterogeneity of microglia in these CNS regions was further suggested by their disparity of plasticity in response to life span progression and HIV-1 pathogenic protein gp120. Our findings indicate that microglia in different CNS compartments are adapted to their local environments to fulfill region-specific biological functions.Graphical abstractDisplay OmittedHighlights•Mouse cortical and spinal microglia are different in their subtype ratios•Cortical and spinal microglia differentiate differently during age progression•Cortical and spinal microglia differentiate differently in HIV-1 gp120 miceImmunology; Developmental Biology; Transcriptomics