文章基本信息

标题：Chromosomes distribute randomly to, but not within, human neutrophil nuclear lobes
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作者：Christine R. Keenan ; Michael J. Mlodzianoski ; Hannah D. Coughlan 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：3
页码：1-24
DOI：10.1016/j.isci.2021.102161
语种：English
出版社：Elsevier
摘要：SummaryThe proximity pattern and radial distribution of chromosome territories within spherical nuclei are random and non-random, respectively. Whether this distribution pattern is conserved in the partitioned or lobed nuclei of polymorphonuclear cells is unclear. Here we use chromosome paint technology to examine the chromosome territories of all 46 chromosomes in hundreds of single human neutrophils – an abundant and famously polymorphonuclear immune cell. By comparing the distribution of chromosomes to randomly shuffled controls and validating with orthogonal chromosome conformation capture technology, we show for the first time that human chromosomes randomly distribute to neutrophil nuclear lobes, while maintaining a non-random radial distribution within these lobes. Furthermore, we demonstrate that chromosome length correlates with three-dimensional volume not only in neutrophils but other human immune cells. This work demonstrates that chromosomes are largely passive passengers during the neutrophil lobing process but are able to subsequently maintain their macro-level organization within lobes.Graphical abstractDisplay OmittedHighlights•Human chromosomes randomly distribute to neutrophil nuclear lobes•However, they sustain a non-random radial distribution within these lobes•Chromosome length correlates with volume in neutrophils and other human immune cells•Gene-regulatory transchromosomal interactions are unlikely in human neutrophilsImmunology; optical imaging; chromosome organization; organizational aspects of cell biology