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标题：Effect of Sericin Supplementation in Collection Medium on Bovine Oocyte Nuclear Maturation
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作者：F A Satrio ; N W K Karja ; M A Setiadi 等
期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2020
卷号：478
期号：1
DOI：10.1088/1755-1315/478/1/012006
语种：English
出版社：IOP Publishing
摘要：Sericin is a water-soluble globular protein derived from silkworm Bombyx mori and has the competency as an antioxidant. This study was conducted to examine the effect of sericin supplementation in the collection medium on bovine oocyte nuclear maturation. Sericin with different concentration (0 (control), 0.1 %, 0.5 %, and 1%) was added to collection medium and maturated for 24 hour at 38.5 °C in 5% of CO2 air. Matured oocytes were stained with acetic-orcein and determined the oocyte nuclear stage under a stereomicroscope. After in vitro maturation, 74-87% of oocytes were reached nuclear maturation (metaphase II). The maturation rates of oocytes were significantly higher at 0.1% group (87.7%) (P<0.05) compared to other groups. There was no significant differences were observed between control group (74.6%), 0.5% group (79.4%), and 1% group (78.3%) (P>0.05). These findings showed that supplementation of 0.1% sericin in the collection medium improved the nuclear maturation of bovine oocytes.