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标题：Screening of antivibrio-producing lactic acid bacteria originated from aquatic animals as probiotic candidates
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作者：M Amin ; M A Liliyanti ; N H Nufus 等
期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2020
卷号：441
期号：1
DOI：10.1088/1755-1315/441/1/012092
语种：English
出版社：IOP Publishing
摘要：Acute hepatopancreatic necrosis disease has been a major challenge faced by white shrimp farmers in many shrimp-producing countries inducing Indonesia. The etiological agent of this disease was a virulent strain of Vibrio parahaemolyticus. However, a technology to treat or to prevent the disease infection has not been established yet, and probiont could be a potential approach for this disease. Thus, this study aimed at screening 35 lactic acid bacteria (LAB) that originated from the gastrointestinal tract of wild white shrimp for the production of antivibrio compounds using an agar well diffusion. The result showed that three LAB strains (LAB-1, LAB-2, and LAB-3) showed antivibrio activity indicated by the formation of clearance zone (diameter of >10mm) on the diffusion agar plate. When the extracellular products (ECP) were incubated with proteinase K, the antivibrio activity was disappeared which indicated that the antimicrobial compounds were bacteriocin-like inhibitory substances (BLIS). Phenotypic studies suggest that the LAB were Labctobacillus sp. (LAB-1 and LAB-2), and Enterococcus sp. (LB-3). These results suggest these LAB are potential candidates for preventing V. parahaemolyticus. Infection, although, further assays by in vivo using live white shrimp, are still required.