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标题：Identification and Expression of TOP3α in Gerbera hybrida
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作者：Shenchong Li ; Ying Cheng ; Dan Sun 等
期刊名称：Horticultural Plant Journal
印刷版ISSN：2468-0141
出版年度：2021
卷号：7
期号：2
页码：167-173
DOI：10.1016/j.hpj.2020.05.002
语种：English
出版社：Elsevier
摘要：AbstractMeiotic recombination and the resulting novel allele combinations are fundamental to plant breeding and the identification of superior hybrids. However, the rate of meiotic crossovers is naturally suppressed below its potential ability, which prevents plant breeding efficiency. Nine suppressors of meiotic recombination have been identified in the model plantArabidopsisand in other crop species. Mutations in these genes can lead to increased recombination frequency and could therefore potentially be used to create hyper-recombinant lines for ornamental breeding. InGerbera hybrida, the anti-crossover factors remain elusive. In this study, we isolated and clonedTOP3αfrom flower buds ofG. hybrida, and it encoded 935 amino acids with three conserved domains TOPRIM, TOP1Ac and zf-GR. Moreover,TOP3αwas the highest expressed at the flower bud stage, which coincided with the occurrence of meiotic recombination, suggesting thatTOP3αis associated with the regulation of meiotic recombination inG. hybrida.
关键词：KeywordsGerbera hybridameiotic recombinationTOP3αgene expressionornamental breeding