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标题：Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes
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作者：Wei Guo ; Zhuoqi Liu ; Jiaqi Yu 等
期刊名称：Horticultural Plant Journal
印刷版ISSN：2468-0141
出版年度：2021
卷号：7
期号：2
页码：142-148
DOI：10.1016/j.hpj.2020.08.003
语种：English
出版社：Elsevier
摘要：AbstractHenosepilachna vigintioctopunctatais an emerging horticultural pest of Solanaceae plants in Asia. Here, we employed transcriptome sequencing of three female and three maleH. vigintioctopunctatalibraries to identify sex-related genes. We recorded 281 599 294 bp of clean reads, andde novoassembly generated 85 206 unigenes, with N50of 1 311 bp and average length of 763 bp. We found 5 002 genes highly expressed in males and 2 179 genes highly expressed in females. Expression profiles of six unigenes specific to females, six unigenes specific to males, and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR; results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis. Using all unigenes as references, we identified 6 657 putative simple sequence repeats. The transcriptome sequences and gene expression profiles of female and maleH. vigintioctopunctatahelp explore genetic mechanisms of sexual dimorphism.
关键词：KeywordsHenosepilachna vigintioctopunctatasexRNA-seqtranscriptome annotationdifferential expression