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标题：TrIPP—a method for tracking the inheritance patterns of proteins in living cells—reveals retention of Tup1p, Fpr4p, and Rpd3L in the mother cell
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作者：Morgane Auboiron ; Pauline Vasseur ; Saphia Tonazzini 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：2
页码：1-32
DOI：10.1016/j.isci.2021.102075
语种：English
出版社：Elsevier
摘要：SummaryInheritance of chromatin-bound proteins theoretically plays a role in the epigenetic transmission of cellular phenotypes. Protein segregation during cell division is however poorly understood. We now describe TrIPP (Tracking the Inheritance Patterns of Proteins): a live cell imaging method for tracking maternal proteins during asymmetric cell divisions of budding yeast. Our analysis of the partitioning pattern of a test set of 18 chromatin-associated proteins reveals that abundant and moderately abundant maternal proteins segregate stochastically and symmetrically between the two cells with the exception of Rxt3p, Fpr4p, and Tup1p, which are preferentially retained in the mother. Low abundance proteins also tend to be retained in the mother cell with the exception of Sir2p and the linker histone H1. Our analysis of chromatin protein behavior in single cells reveals potentially general trends such as coupled protein synthesis and decay and a correlation between protein half-lives and cell-cycle duration.Graphical abstractDisplay OmittedHighlights•Most protein half-lives are equal to cell-cycle length•Net synthesis rates of 18 test proteins are 2-fold higher than their decay rates•Most tested proteins segregate stochastically between mothers and daughters•“Old” Rxt3p, Tup1p, and Fpr4p and low abundance proteins are retained in the motherBiological sciences; molecular biology; cell biology