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标题：The role of Piezo1 in conventional aqueous humor outflow dynamics
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作者：Wei Zhu ; Fei Hou ; Jingwang Fang 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：2
页码：1-33
DOI：10.1016/j.isci.2021.102042
语种：English
出版社：Elsevier
摘要：SummaryControlling intraocular pressure (IOP) remains the mainstay of glaucoma therapy. The trabecular meshwork (TM), the key tissue responsible for aqueous humor (AH) outflow and IOP maintenance, is very sensitive to mechanical forces. However, it is not understood whether Piezo channels, very sensitive mechanosensors, functionally influence AH outflow. Here, we characterize the role of Piezo1 in conventional AH outflow. Immunostaining and western blot analysis showed that Piezo1 is widely expressed by TM. Patch-clamp recordings in TM cells confirmed the activation of Piezo1-derived mechanosensitive currents. Importantly, the antagonist GsMTx4 for mechanosensitive channels significantly decreased steady-state facility, yet activation of Piezo1 by the specific agonist Yoda1 did not lead to a facility change. Furthermore, GsMTx4, but not Yoda1, caused a significant increase in ocular compliance, a measure of the eye's transient response to IOP perturbation. Our findings demonstrate a potential role for Piezo1 in conventional outflow, likely under pathological and rapid transient conditions.Graphical abstractDisplay OmittedHighlights•Piezo1 is functionally expressed in the TM, the most important tissue controlling IOP•Suppression of mechanosensitive channel leads to a significant decrease in facility•Our data suggest a role for Piezo in pathological situations and rapid IOP transientsOphthalmology; Cellular Physiology; Biomechanics