文章基本信息

标题：Systematic errors in orthology inference and their effects on evolutionary analyses
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作者：Paschalis Natsidis ; Paschalia Kapli ; Philipp H. Schiffer 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：2
页码：1-18
DOI：10.1016/j.isci.2021.102110
语种：English
出版社：Elsevier
摘要：SummaryThe availability of complete sets of genes from many organisms makes it possible to identify genes unique to (or lost from) certain clades. This information is used to reconstruct phylogenetic trees; identify genes involved in the evolution of clade specific novelties; and for phylostratigraphy—identifying ages of genes in a given species. These investigations rely on accurately predicted orthologs. Here we use simulation to produce sets of orthologs that experience no gains or losses. We show that errors in identifying orthologs increase with higher rates of evolution. We use the predicted sets of orthologs, with errors, to reconstruct phylogenetic trees; to count gains and losses; and for phylostratigraphy. Our simulated data, containing information only from errors in orthology prediction, closely recapitulate findings from empirical data. We suggest published downstream analyses must be informed to a large extent by errors in orthology prediction that mimic expected patterns of gene evolution.Graphical AbstractDisplay OmittedHighlights•Presence of shared orthologs across species is used for evolutionary analyses•We simulated realistic sets of orthologs with no gains or losses•Errors predicting shared orthologs correlate with phylogenetic relationships•Presence/absence datasets based on errors recapitulate findings from empirical dataBiological Sciences; Evolutionary Biology; Evolutionary Processes; Evolutionary Mechanisms; Phylogenetics; Phylogeny