文章基本信息

标题：Screening of six medicinal plant species for antileishmanial activity
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作者：Fabiana Borges Padilha Ferreira ; Márcia Regina Pereira Cabral ; Maria Helena Sarragiotto 等
期刊名称：Acta Pharmaceutica
电子版ISSN：1846-9558
出版年度：2021
卷号：71
期号：3
页码：399-414
DOI：10.2478/acph-2021-0023
语种：English
出版社：Sciendo
摘要：This study is aimed to investigate thein vitroanti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely,Nectandra megapotamica,Brunfelsia uniflora,Myrcianthes pungens,Anona muricata,Hymenaea stigonocarpaandPiper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms ofLeishmania amazonensisandLeishmania braziliensisand the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results forL. amazonensisandL. braziliensiswere shown by the ethanolic extract of the fruits ofHymenaea stigonocarpaand dichloromethane extract of the roots ofPiper corcovadensis, withIC50of 160 and 150 μg mL−1, resp. Ethanolic extracts ofA. muricata(leaf),B. uniflora(flower and leaf),M. pungens(fruit and leaf),N. megapotamica(leaf), and aqueous extract ofH. stigonocarpa(fruit) showedIC50> 170 μg mL−1forL. amazonensisand > 200 μg mL−1forL. braziliensis. The extracts exhibited low cytotoxicity towards J774.A1 macrophages withCC50> 1000 μg mL−1and hemolytic activity from 0 to 46.1 %.