文章基本信息

标题：Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging
本地全文：下载
作者：Giorgia Zambito ; Mary P. Hall ; Monika G. Wood 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：1
页码：1-20
DOI：10.1016/j.isci.2020.101986
语种：English
出版社：Elsevier
摘要：SummaryForin vivomulticolor bioluminescence applications, red and near-infrared signals are desirable over shorter wavelength signals because they are not as susceptible to light attenuation by blood and tissue. Herein, we describe the development of a new click beetle luciferase mutant, CBG2, with a red-shifted color emission. When paired with NH2-NpLH2 luciferin, CBG2 (λ = 660 nm) and CBR2 (λ = 730 nm) luciferases can be used for simultaneous dual-color bioluminescence imaging in deep tissue. Using a spectral unmixing algorithm tool it is possible to distinguish each spectral contribution. Ultimately, this enzyme pair can expand the near-infrared bioluminescent toolbox to enable rapid visualization of multiple biological processes in deep tissue using a single substrate.Graphical AbstractDisplay OmittedHighlights•CBG2 is a new click beetle mutant derived from CBG99 luciferase•It has a far red-shifted bioluminescent emission with NH2-NpLH2 luciferin (λ = 660 nm)•CBG2 with CBR2 luciferase and NH2-NpLH2 allows fast acquisition of data in deep tissueOptical Imaging; Biological Services; Biophysics