文章基本信息

标题：Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses
本地全文：下载
作者：Zohreh Farsi ; Marie Walde ; Agnieszka E. Klementowicz 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：1
页码：1-31
DOI：10.1016/j.isci.2020.101909
语种：English
出版社：Elsevier
摘要：SummaryMammalian central synapses exhibit vast heterogeneity in signaling strength. To understand the extent of this diversity, how it is achieved, and its functional implications, characterization of a large number of individual synapses is required. Using glutamate imaging, we characterized the evoked release probability and spontaneous release frequency of over 24,000 individual synapses. We found striking variability and no correlation between action potential-evoked and spontaneous synaptic release strength, suggesting distinct regulatory mechanisms. Subpixel localization of individual evoked and spontaneous release events reveals tight spatial regulation of evoked release and enhanced spontaneous release outside of evoked release region. Using on-stage post hoc immune-labeling of vesicle-associated proteins, Ca2+-sensing proteins, and soluble presynaptic proteins we were able to show that distinct molecular ensembles are associated with evoked and spontaneous modes of synaptic release.Graphical abstractDisplay OmittedHighlights•Glutamate imaging reveals high variability in release properties between synapses•Evoked and spontaneous synaptic release are regulated by distinct mechanisms•Spontaneous release is enhanced outside of evoked release region at the synapse•Distinct molecular ensembles contribute to spontaneous and evoked releaseOptical Imaging ; Molecular Neuroscience; Cellular Neuroscience