文章基本信息

标题：Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells
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作者：Tomokazu Fukuda ; Kai Furuya ; Kouhei Takahashi 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2021
卷号：24
期号：1
页码：1-82
DOI：10.1016/j.isci.2020.101929
语种：English
出版社：Elsevier
摘要：SummaryThe immortalized cell is an essential research tool that uses robust growth properties for the functional investigation of gene products. Immortalized mammalian cells have mainly been established using three methods: expression of simian vacuolating virus 40 T antigen (the SV40 method); human papilloma virus-derived oncoprotein E6/E7 (the E6/E7 method); or combinatorial expression of R24C mutant cyclin-dependent kinase 4, cyclin D1, and telomerase reverse transcriptase (the K4DT method). However, it is unclear as to which method is optimal for anin vitromodel. Here, we compared the biological characteristics and genome-wide expression profiles of immortalized human dermal papilla cells generated by the SV40, E6/E7, or K4DT method. To our knowledge, this is the first study to comprehensively compare expression profiles to determine the optimal immortalization method for maintaining the original nature of the wild-type cells. These data would be valuable to scientists aiming to establish new immortalized cell lines.Graphical AbstractDisplay OmittedHighlights•Expression profiles of immortalized dermal papilla cells by the SV40, E6/E7, K4DT method were analyzed•The distance from wild type to K4DT was close, E6E7 was middle, SV40 was most distant•K4DT method showed the most conservative profile when it compared with SV40 and E6E7 methodCell Biology; Stem Cell Research; Technical Aspects of Cell Biology