摘要:The development of the bionic water channel aims to replace the possible use of natural aquaporins (AQPs) for water purification, while retaining the ability of natural AQPs to carry out ultra-fast water transport and repel ions. Carbon nanotube channels (CNTCs) are a convenient membrane-based model system for studying nano-fluidic transport that replicates a number of key structural features of biological membrane channels. In this report, we describe protocols for CNTCs synthesis by ultrasound-assisted cutting of long CNTs in the presence of lipid amphiphiles. CNTCs have a similar thickness to the lipid membrane and high affinity for it. The ultra-short high-affinity CNTCs have high permeability and ion selectivity. The water permeability of the CNTCs is 1936 ± 123 μm/s, which is 2.3 times that of natural AQPs, and completely rejects salt ions. In general, carbon nanotubes represent a multifunctional nanopore building module for creating high-ranking functional bionic materials. This study has reference significance for the design of new bionic water channel and the actual development of bionic membrane based on CNTs.