文章基本信息

标题：Response Surface Methodology to Optimize Degradation on Phenol by Pseudomonas stutzeri ZH-1
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作者：Yong Yan ; Qingping Hu
期刊名称：Open Access Library Journal
印刷版ISSN：2333-9705
电子版ISSN：2333-9721
出版年度：2020
卷号：07
期号：11
页码：1-15
DOI：10.4236/oalib.1106952
语种：English
出版社：Scientific Research Pub
摘要：In order to obtain the optimal conditions for the degradation of phenol by Pseudomonas stutzeri ZH-1, based on the single factor experiment, the Box Behnken design test of Response Surface Method was used. Four variables of glucose concentration (0.4, 0.6, 0.8 g/L), inoculation concentration (3%, 5%, 7%), pH (6, 7, 8) and temperature (35°C, 37.5°C, 40°C) were used to identify the significant effects and interactions in the batch studies. A second order polynomial regression model, has been developed using the experimental data. The experimental values were in good agreement with the predicted values, and the correlation coefficient was found to be 0.9901. Optimum conditions of the variables for the growth of Pseudomonas stutzeri ZH-1 and for maximum biodegradation of phenol are glucose concentration (0.7 g/L), inoculation concentration 5%, pH = 7 and temperature 37°C with maximum phenol degradation rate of 89.62%.
关键词：Response Surface MethodologyBox-BehnkenPhenol DegradationPseudomonas stutzeri