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标题：Design of Multi-Epitope Vaccine against SARS-CoV-2
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作者：Irini Doytchinova ; Andrey Tchorbanov
期刊名称：Cybernetics and Information Technologies
印刷版ISSN：1311-9702
电子版ISSN：1314-4081
出版年度：2020
卷号：20
期号：6
页码：185-193
DOI：10.2478/cait-2020-0072
语种：English
出版社：Bulgarian Academy of Science
摘要：The ongoing COVID-19 pandemic requires urgently specific therapeuticsand approved vaccines. Here, the four structural proteins of the Severe AcuteRespiratory Syndrome CoronaVirus 2 (SARS-CoV-2), the causative agent of COVID-19, are screened by in-house immunoinformatic tools to identify peptides acting aspotential T-cell epitopes. In order to act as an epitope, the peptide should beprocessed in the host cell and presented on the cell surface in a complex with theHuman Leukocyte Antigen (HLA). The aim of the study is to predict the bindingaffinities of all peptides originating from the structural proteins of SARS-CoV-2 to30 most frequent in the human population HLA proteins of class I and class II and toselect the high binders (IC 50 < 50 nM). The predicted high binders are compared toknown high binders from SARS-CoV conserved in CoV-2 and 77% of them coincided.The high binders will be uploaded onto lipid nanoparticles and the multi-epitopevaccine prototype will be tested for ability to provoke T-cell mediated immunity andprotection against SARS-CoV-2.
关键词：COVID-19; multi-epitope vaccine; SARS-CoV; SARS-CoV-2; EpiJen;EpiTOP; EpiDOCK; high binders; HLA class I; HLA class II .