文章基本信息

标题：Quantitative of progesterone using isotope dilution-matrix-assisted laser desorption ionization-time of flight mass spectrometry
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作者：Ming-Hui Yang ; Han-Ping You ; Hsin-Yi Wu 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2020
卷号：7
页码：1-4
DOI：10.1016/j.mex.2020.100812
语种：English
出版社：Elsevier
摘要：AbstractA quantification assay based on isotope dilution mass spectrometry to determine the concentration of progesterone in human serum was reported. Incorporated with13C3-progesterone, serum samples were subjected to progesterone extraction and clean-up by C4 solid-phase-extraction columns and hexane-based liquid/liquid extraction, respectively. The cleaned-up serum samples were then subjected to MALDI-TOF mass spectrometry for the quantification of progesterone. In the study, the recovered progesterone concentration determined by the assay showed good robustness and constancy in comparison to conventional radioimmunologic assay. We concluded that the13C3-progesterone-based quantification assay is a robust method for the measurement of serum progesterone.Advantages of this technique includes:• This study describes a MALDI-TOF/MS method for the determination of serum progesterone.• The technique is simple and easy to apply on MALDI-TOF/MS for serum progesterone analysis.• The correlation coefficient between MALDI-TOF MS and RIA was 0.981 for serum progesterone.Graphical abstractDisplay Omitted
关键词：MALDI-TOF/MS;Progesterone;Isotope dilution