文章基本信息

标题：Avidin-biotin technology to synthesize multi-arm nano-construct for drug delivery
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作者：Chenzhen Zhang ; Tengfei He ; Armin Vedadghavami 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2020
卷号：7
页码：1-14
DOI：10.1016/j.mex.2020.100882
语种：English
出版社：Elsevier
摘要：AbstractHere we describe methods for synthesizing a cationic, multi-arm Avidin (mAv) nano-construct that has a wide range of applications in drug delivery and imaging of negatively charged tissues. We use Avidin-biotin technology that gives the flexibility for conjugating biotinylated Dexamethasone to mAv by simple mixing at room temperature. We also describe methods to control hydrolysis rates of ester linkers to enable sustained (and tunable) drug release rates in therapeutic doses.•Multi-arm structure provides multiple sites for covalent conjugation of drugs•Use of Avidin-biotin reaction gives multi-arm nano-construct a modular design enabling conjugation and delivery of similar sized biotinylated drugs.Graphical abstractDisplay Omitted
关键词：Avidin;Biotin;PEGs;Ester linkers;Negatively charged tissues;Drug delivery