文章基本信息

标题：Standardized protocol for determination of biohydrogen potential
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作者：Julián Carrillo-Reyes ; Germán Buitrón ; Iván Moreno-Andrade 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2020
卷号：7
页码：1-12
DOI：10.1016/j.mex.2019.11.027
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractBiohydrogen production potential (BHP) depends on several factors like inoculum source, substrate, pH, among many others. Batch assays are the most common strategy to evaluate such parameters, where the comparison is a challenging task due to the different procedures used. The present method introduces the first internationally validated protocol, evaluated by 8 independent laboratories from 5 different countries, to assess the biohydrogen potential. As quality criteria, a coefficient of variation of the cumulative hydrogen production (Hmax) was defined to be <15 %. Two options to run BHP batch tests were proposed; a manual protocol with periodic measurements of biogas production, needing conventional laboratory materials and analytical equipment for biogas characterization; and an automatic protocol, which is run in a device developed for online measurements of low biogas production. The detailed procedures for both protocol options are presented, as well as data validating them. The validation showed acceptable repeatability and reproducibility, measured as intra- and inter-laboratory coefficient of variation, which can be reduced up to 9 %.
关键词：Automatic protocol;Dark fermentation;Heat-treated inoculum;Manual protocol