文章基本信息

标题：A protocol of rapid laboratory evolution by genome shuffling in Kluyveromyces marxianus
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作者：Li Wu ; Mengzhu Wang ; Genhan Zha 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2020
卷号：7
页码：1-5
DOI：10.1016/j.mex.2020.101138
语种：English
出版社：Elsevier
摘要：AbstractGenome shuffling is a process to combine advantage traits by the recombination of the entire genome and it has been successfully applied in the laboratory evolution of various industrial microorganisms. However, genome shuffling has not been described inKluyveromyces marxianus(KM), a promising yeast host for the expression of heterologous proteins. In this protocol, genome shuffling in KM is performed by sexual reproduction and is combined with high-throughput screening to obtain high-yielding strains. Notably, the screening of diploid clones risen from one mating mixture is carried out to improve the effectiveness of evolution. Mating-sporulation-mating cycles are repeated to obtain KM strain with ideal traits.•The method combines genome shuffling with high-throughput to achieve strains displaying high yielding of heterologous proteins.•This method can be applied to the genome shuffling of other species when only a few starting strains are available for sexual reproduction.Graphical abstractDisplay Omitted
关键词：Genome shuffling;Mating;Ideal phenotypes