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标题：Amino-acid amendment of Arginine-325-Tryptophan in rs13266634 genetic polymorphism studies of the SLC30A8 gene with type 2 diabetes-mellitus patients featuring a positive family history in the Saudi population
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作者：Khalid Khalaf Alharbi ; Manal Abudawood ; Imran Ali Khan 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2021
卷号：33
期号：1
页码：1-8
DOI：10.1016/j.jksus.2020.101258
语种：English
出版社：Elsevier
摘要：AbstractType 2 diabetes mellitus (T2DM) is a complex metabolic disorder with chronic hyperglycemia. Genome-wide association studies (GWAS) have identified many genes and, among them, solute carrier family 30 member 8 (SLC30A8) was one of the important genes linked to the development of T2DM risk. The relationship between T2DM and the SLC30A8 gene is linked through zinc, which plays a key role in the storage and secretion of insulin. The rs13266634 polymorphism includes a strong genetic association in case-control andmeta-analysis studies of the global population. The aim of this current study was to scrutinize the genetic relationship between the rs13266634 polymorphism in theSLC30A8gene with T2DM subjects selected with a family history in the Saudi population. This study involved 120 cases of diagnosed T2DM and 120 confirmed healthy controls that were recruited to screen rs13266634 polymorphisms through a genotyping analysis followed by PCR and RFLP analysis. Baseline characteristics between cases and controls have been evaluated with Student’st-test. The study results confirmed the genetic association between the allele (p = 0.001), genotypes (CT = 0.005 and TT = 0.03), and various genetic patterns of inheritance (p = 0.001 and p = 0.02). Both analysis of variance (ANOVA) and binary logistic regression analysis revealed non-signiifcant association with T2DM cases and biochemical parameters (p > 0.05). In conclusion, the current results have confirmed the strong genetic association between T2DM cases and controls in the Saudi population with rs13266634 polymorphisms of theSLC30A8gene.
关键词：Type 2 diabetes mellitus;SLC30A8-gene;Rs13266634 polymorphism;Genotyping