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标题：Purification and characterization of carboxymethylcellulase from Bacillus pumilus EWBCM1 isolated from earthworm gut ( Eudrilus eugeniae)
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作者：Thiruvengadam Shankar ; Subbiah Sankaralingam ; Chellapandian Balachandran 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2021
卷号：33
期号：1
页码：1-8
DOI：10.1016/j.jksus.2020.101261
语种：English
出版社：Elsevier
摘要：AbstractThe present work describes purification and characterization of an extracellular cellulase from a newly isolated cellulolytic bacterial strainBacillus pumilusEWBCM1. The candidate strain was tested for its abilities to hydrolyze the structural polysaccharides through the depolymerising activities of carboxymethylcellulase. The purification of cellulase was carried out by ammonium sulphate precipitation, DEAE cellulose and sephadex G-100. Purified cellulase was found to be 47.56 fold along with 1.46 U/mg of protein and the molecular weight of cellulase was 47 kDa through SDS-PAGE electrophoresis. The optimum activity of cellulase was registered at pH 10 at 50 °C. In the present study, metal ions inferred the addition of 5 mM CaCl2(128%) was recorded good activity than the other tested metal ions. Maximum activity of purified cellulase was recorded in 3% of NaCl and 5% of surfactants medium. The bioactive of purified cellulase was carried out with various commercial detergent and antimicrobial activity.
关键词：Purification;Characterization;SDS-PAGE;CMCase;Bacillus pumilus