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标题：Erythroid induction activity of Aquilegia fragrans and Aquilegia pubiflora and identification of compounds using liquid chromatography-tandem mass spectrometry
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作者：Nudrat Aziz ; Muhammad Noman Khan ; Faraz Ul Haq 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2021
卷号：33
期号：1
页码：1-10
DOI：10.1016/j.jksus.2020.10.024
语种：English
出版社：Elsevier
摘要：AbstractAquilegia fragrans(AF) andAquilegia pubiflora(AP) are the two medicinally important species of genusAquilegiaused for the treatment of various diseases and infections. This paper describes the potential of fetal hemoglobin induction activity of the methanolic extracts of AF and AP in K562 cell line. AF and AP have shown 27.147 ± 1.376 and 32.786 ± 1.048 percent erythroid induction, respectively at 15.625 (µg/mL) concentration which suggested that both plants can be the source of potential fetal hemoglobin inducers and may be used for the treatment of β-thalassemia. Phytochemical analyses of both species were also evaluated by using high-resolution LC-ESI-QTOF-MS/MS techniques. A Total of thirty compounds were identified using positive and negative ionization modes. The identification was based on the matching of high-resolution masses, isotopic pattern, and MS/MS fragmentation. Several statistical analyses were performed to evaluate the distribution of compounds in both species. Identified compounds belong to various classes including flavonoids, steroids, lignans, terpenoids, benzofuran and coumarins. The established chemical fingerprints will be helpful in standardization and quality control of plant extracts.
关键词：Aquilegia pubiflora;Aquilegia fragrans;β-thalassemia;Fetal hemoglobin