文章基本信息

标题：Honey bee gut an unexpected niche of human pathogen
本地全文：下载
作者：Syed Ishtiaq Anjum ; Fahad Aldakheel ; Ayesha Haleem Shah 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2021
卷号：33
期号：1
页码：1-5
DOI：10.1016/j.jksus.2020.101247
语种：English
出版社：Elsevier
摘要：AbstractThe gut microbiota of honey bees (Apis mellifera) can be symbiotic or pathogenic and therefore, important for bee survival and honey production. To study gut cultivable bacteria of honey bees, 30 honey bee samples were collected from district Kohat of Khyber Pakhtunkhwa Province as there is no information about the diversity of bee gut microbiota from Pakistan. Complete digestive system of the worker bee was dissected and processed for bacterial isolation. A total of 219 bacteria were obtained and characterized by bacterialogical parameters. The human pathogenic bacterial isolates were identified and confirmed on 16S ribosomal DNA sequencing. Combined microbiological practices revealed the presence of following bacterial genera:Bacillus, Enterococcus, Escherichia, Micrococcus, Morganella, Ochrobactrum, Pseudomonas, Salmonella, Shigella,Sphingomonas andStaphylococcus. Two pathogenic bacteriaSalmonella entericaandShigella sonneicausing diseases in man and other animals are confidently characterized. This work suggested that foragerApis melliferagut acts as reservoir and potential vector of bacterial pathogens.
关键词：Apis mellifera;Bacterial flora;Colony PCR;16S rDNA sequencing