文章基本信息

标题：Transcriptome dataset of human corneal endothelium based on ribosomal RNA-depleted RNA-Seq data
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作者：Yuichi Tokuda ; Naoki Okumura ; Yuya Komori 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2020
卷号：7
期号：1
页码：1-6
DOI：10.1038/s41597-020-00754-1
语种：English
出版社：Nature Publishing Group
摘要：The corneal endothelium maintains corneal transparency; consequently, damage to this endothelium by a number of pathological conditions results in severe vision loss. Publicly available expression databases of human tissues are useful for investigating the pathogenesis of diseases and for developing new therapeutic modalities; however, databases for ocular tissues, and especially the corneal endothelium, are poor. Here, we have generated a transcriptome dataset from the ribosomal RNA-depleted total RNA from the corneal endothelium of eyes from seven Caucasians without ocular diseases. The results of principal component analysis and correlation coefficients (ranged from 0.87 to 0.96) suggested high homogeneity of our RNA-Seq dataset among the samples, as well as sufficient amount and quality. The expression profile of tissue-specific marker genes indicated only limited, if any, contamination by other layers of the cornea, while the Smirnov-Grubbs test confirmed the absence of outlier samples. The dataset presented here should be useful for investigating the function/dysfunction of the cornea, as well as for extended transcriptome analyses integrated with expression data for non-coding RNAs.