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标题：Isolation and Characterization of Hexadecane Degrading Bacteria from Oil- polluted soil in Gio Community, Niger Delta, Nigeria
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作者：A.U. Okoye ; C.B. Chikere ; G.C. Okpokwasili 等
期刊名称：Scientific African
印刷版ISSN：2468-2276
出版年度：2020
卷号：9
页码：1-9
DOI：10.1016/j.sciaf.2020.e00340
语种：English
出版社：Elsevier
摘要：Highlights•Four individual bacterial isolates had high hexadecane degradation capability.•The existing autochthonous bacteria were metabolically active, with capacity for hexadecane degradation and thus are potential candidates for bioremediation.•The study revealed that the bacteria in the polluted soil were predominantlyPseudomonassp.,Bacillussp.,Achromobactersp. andSerratiasp.AbstractThis study investigated the presence of viable indigenous hexadecane degraders in chronically polluted soil in Gio community, Niger Delta. Crude oil-polluted samples were collected from the four GPS coordinate points at depth of 0-0.15m (Gio polluted surface soil [GPS]), 1m (Gio polluted subsurface soil [GPSS]) respectively and Gio unpolluted control soil (GUPS) was collected from approximately 1000m away from the polluted site. Gas chromatography - flame ionization detector (GC-FID) analysis for total petroleum hydrocarbon (TPH) were 36,776 ppm, 14,100 ppm, 480 ppm for GPS, GPSS and GUPS respectively. Polycyclic aromatic hydrocarbon (PAHs) concentrations determined were 12,210 ppm, 3,250 ppm, 23 ppm for GPS, GPSS and GUPS respectively. Total cultivable hydrocarbon utilizing bacterial count (TCHUB) for GPS, GPSS and GUPS were 6.6 × 105cfu/g, 6.4 × 105cfu/g and 3.96 × 104cfu/g respectively. Out of the 22 bacteria isolated and characterized from the crude oil enrichment method,Pseudomonassp. (65 %),Bacillussp. (66%),Achromobactersp.Proteussp.(45%) andSerratiasp (40%). demonstrated higher biodegradation potential for hexadecane using 2,6-dichlorophenol indophenols (DCPIP) redox indicator. In conclusion the extant autochthonous bacteria are metabolically active, can effectively degrade hexadecane and could be good candidates for bioremediation.
关键词：KeywordsCharacterizationHexadecaneBacteriaOil-pollutedGio communityNiger Delta