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标题：Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma b rucei
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作者：K. Shanmugha Rajan ; Tirza Doniger ; Smadar Cohen-Chalamish 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：12
页码：1-45
DOI：10.1016/j.isci.2020.101780
语种：English
出版社：Elsevier
摘要：SummaryThe parasiteTrypanosoma bruceiis the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action oftrans-splicing and polyadenylation. Regulation of mRNA function is mediated mainly by RNA binding proteins affecting mRNA stability and translation. In this study, we describe the identification of 62 non-coding (nc) RNAs that are developmentally regulated and/or respond to stress. We characterized two novel anti-sense RNA regulators (TBsRNA-33 and 37) that originate from the rRNA loci, associate with ribosomes and polyribosomes, and interactin vivowith distinct mRNA species to regulate translation. Thus, this study suggests for the first-time anti-sense RNA regulators as an additional layer for controlling gene expression in these parasites.Graphical AbstractDisplay OmittedHighlights•Trypanosome non-coding RNAs (ncRNAs) are developmentally regulated during cycling between two hosts•ncRNAs originate from rRNA locus and associate with the ribosomeen routeto cytoplasm•In vivocross-linking enable identification of target RNA species regulated by ncRNAs•Trypanosomes possess anti-sense ncRNAs that regulate translationMolecular Biology; Omics