文章基本信息

标题：Thermodynamic Surface Analyses to Inform Biofilm Resistance
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作者：T. Brian Cavitt ; Jasmine G. Carlisle ; Alexandra R. Dodds 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：11
页码：1-38
DOI：10.1016/j.isci.2020.101702
语种：English
出版社：Elsevier
摘要：SummaryBiofilms are the habitat of 95% of bacteria successfully protecting bacteria from many antibiotics. However, inhibiting biofilm formation is difficult in that it is a complex system involving the physical and chemical interaction of both substrate and bacteria. Focusing on the substrate surface and potential interactions with bacteria, we examined both physical and chemical properties of substrates coated with a series of phenyl acrylate monomer derivatives. Atomic force microscopy (AFM) showed smooth surfaces often approximating surgical grade steel. Induced biofilm growth of five separate bacteria on copolymer samples comprising varying concentrations of phenyl acrylate monomer derivatives evidenced differing degrees of biofilm resistance via optical microscopy. Using goniometric surface analyses, the van Oss-Chaudhury-Good equation was solved linear algebraically to determine the surface energy profile of each polymerized phenyl acrylate monomer derivative, two bacteria, and collagen. Based on the microscopy and surface energy profiles, a thermodynamic explanation for biofilm resistance is posited.Graphical AbstractDisplay OmittedHighlights•Surface energy components affect bacterial adhesion to substrates•Bacterial adhesion is directly related to the substrate's polar component•Bacterial adhesion is inversely related to the substrate's base component•Polarizable substrates may allow bacterial association, and not adhesionPolymer Chemistry; Thermodynamics; Microbiology