文章基本信息

标题：Identification of a Quorum Sensing-Dependent Communication Pathway Mediating Bacteria-Gut-Brain Cross Talk
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作者：Friederike Uhlig ; Luke Grundy ; Sonia Garcia-Caraballo 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2020
卷号：23
期号：11
页码：1-30
DOI：10.1016/j.isci.2020.101695
语种：English
出版社：Elsevier
摘要：SummaryDespite recently established contributions of the intestinal microbiome to human health and disease, our understanding of bacteria-host communication pathways with regard to the gut-brain axis remains limited. Here we provide evidence that intestinal neurons are able to “sense” bacteria independently of the host immune system. Using supernatants from cultures of the opportunistic pathogenStaphylococcus aureus(S.aureus) we demonstrate the release of mediators with neuromodulatory properties at high population density. These mediators induced a biphasic response in extrinsic sensory afferent nerves, increased membrane permeability in cultured sensory neurons, and altered intestinal motility and secretion. Genetic manipulation ofS.aureusrevealed two key quorum sensing-regulated classes of pore forming toxins that mediate excitation and inhibition of extrinsic sensory nerves, respectively. As such, bacterial mediators have the potential to directly modulate gut-brain communication to influence intestinal symptoms and reflex functionin vivo, contributing to homeostatic, behavioral, and sensory consequences of infection.Graphical AbstractDisplay OmittedHighlights•Quorum sensing-regulated secretions modulate intestinal neurons, secretion, and motility•Intestinal neurons are able to “sense” bacteria independently of the host immune system•Excitation and inhibition of intestinal afferents is mediated by pore-forming toxins•Bacterial mediators directly influence microbiota-gut-brain axis signaling pathwaysNeuroscience; Microbiology