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标题：Anti-biofilm compound of 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane from marine Nocardiopsis sp. DMS 2 (MH900226) against biofilm forming K. pneumoniae
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作者：Govindan Nadar Rajivgandhi ; Govindan Ramachandran ; C. Chenthis Kanisha 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2020
卷号：32
期号：8
页码：3495-3502
DOI：10.1016/j.jksus.2020.10.012
语种：English
出版社：Elsevier
摘要：AbstractIn this study, the 16S rRNA of the marine endophytic actinomycete was characterized and identified asNocardiopsis sp. strain DMS 2 (MH900226). Partial purification and spectroscopies data were interpreted and confirmed as 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane. Anti-bacterial activity of the crude extract and partially purified compound were shown highest inhibition activity against biofilm formingK. pneumoniae. Minimum biofilm inhibition concentration of identified compound againstK. pneumoniaewas exhibited at the concentration of 300 µg/mL. Decreased metabolic activity and modified exopolysaccharide production of biofilm forming cells were observed at 350 µg/mL and 300 µg/mL concentration. At the same biofilm inhibition concentration, the intracellular architecture of the biofilm cells and extracellular shape modifications were clearly viewed by confocal laser scanning electron microscope and scanning electron microscope respectively. All thein-vitroinhibition results and microscopic images were strongly suggested that the identified compound was a potential anti-biofilm agent against testedK. pneumoniae.
关键词：Marine environment;Endophytes;Nocardiopsis;Minimum biofilm inhibition concentration;Metabolic activity;Intracellular damages